rmif 300-69 (PeproTech)
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rmif 300-69
Rmif 300 69, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rmif+300-69/rmif+300+69/pmc07673366-183-17-19
Average 90 stars, based on 1 article reviews
Rmif 300 69, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rmif+300-69/rmif+300+69/pmc07673366-183-17-19
Average 90 stars, based on 1 article reviews
rmif 300-69 - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling"
Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling
Journal: Biology Open
doi: 10.1242/bio.053447
Figure Legend Snippet: MIF promoted proliferation of BPH-1 and PWR-1E cells. (A) MTT assay showed the proliferation of BPH-1 cells treated with various concentrations of rMIF for 3 days. Data are presented as mean±s.d., n =3. (B) CCK8 assay showed the proliferation of BPH-1 cells treated with control, rMIF (100 ng/ml), rMIF (100 ng/ml)+ISO-1 (10 µM) and ISO-1 (10 µM), respectively. Data are presented as mean±s.d., n =3. (C) CCK8 assay showed the proliferation of PWR-1E cells treated with control, rMIF, rMIF+ISO-1 and ISO-1, respectively. Data are presented as mean±s.d., n =3. (D) JC-1 assay showed the growth rates of the BPH-1 cells treated by control, rMIF, rMIF+ISO-1 and ISO-1, respectively. Data are presented as mean±s.d., n =3. (E) JC-1 assay showed the growth rates of the PWR-1E cells treated by control, rMIF, rMIF+ISO-1 and ISO-1, respectively. Data are presented as mean±s.d., n =3. * P <0.05, * P <0.05. Statistical analyses were performed using ANOVA followed by Tukey's test for multiple comparison.
Techniques Used: MTT Assay, CCK-8 Assay, Control, Comparison
Figure Legend Snippet: Flow cytometry test that MIF promoted proliferation of BPH-1 and PWR-1E cells. (A) Flow cytometry showed cell cycle in BPH-1 cells treated by control, rMIF, rMIF+ISO-1 and ISO-1, respectively. (B) Histogram for S+G2/M phase of the cell cycle results in BPH-1 cells. Data are presented as mean±s.d., n =3. (C) Flow cytometry showed cell cycle in PWR-1E cells treated by control, rMIF, rMIF+ISO-1 and ISO-1, respectively. (D) Histogram for S+G2/M phase of the cell cycle results in PWR-1E cells. Data are presented as mean±s.d., n =3. * P <0.05. Statistical analyses were performed using ANOVA followed by Tukey's test for multiple comparison.
Techniques Used: Flow Cytometry, Control, Comparison
Figure Legend Snippet: MIF modulation COX-2 and P53 signaling pathway. (A) Western blotting detected the expression of COX-2 and P53 in BPH-1 cells treated by control, rMIF and rMIF+ISO-1, respectively. (B) Western blot analysis: the expression of COX-2 and P53 in BPH-1 cells treated by control, rMIF and rMIF+ISO-1. Data are presented as mean±s.d., n =3. (C) Western blotting detected the expression of COX-2 and P53 in PWR-1E cells treated by control, rMIF and rMIF+ISO-1, respectively. (D) Western blot analysis: the expression of COX-2 and P53 in PWR-1E cells treated by control, rMIF and rMIF+ISO-1. Data are presented as mean±s.d., n =3. * P <0.05. Statistical analyses were performed using ANOVA followed by Tukey's test for multiple comparison.
Techniques Used: Western Blot, Expressing, Control, Comparison
Figure Legend Snippet: COX-2 is a key factor of MIF promoted proliferation. (A) CCK8 assay showed the proliferation of BPH-1 cells treated with control, rMIF (100 ng/ml), rMIF (100 ng/ml)+celecoxib (5 µM) and celecoxib (5 µM), respectively. Data are presented as mean±s.d., n =3. (B) CCK8 assay showed the proliferation of PWR-1E cells treated with control, rMIF, rMIF+celecoxib and celecoxib, respectively. Data are presented as mean±s.d., n =3. (C) JC-1 assay showed the growth rates of the BPH-1 cells treated by control, rMIF, rMIF+celecoxib and celecoxib, respectively. Data are presented as mean±s.d., n =3. (D) JC-1 assay showed the growth rates of the PWR-1E cells treated by control, rMIF, rMIF+celecoxib and celecoxib, respectively. Data are presented as mean±s.d., n =3. * P <0.05. Statistical analyses were performed using ANOVA followed by Tukey's test for multiple comparison.
Techniques Used: CCK-8 Assay, Control, Comparison
Figure Legend Snippet: Flow cytometry test that COX-2 is a key factor of MIF promoted proliferation. (A) Flow cytometry showed cell cycle in BPH-1 cells treated by control, rMIF, rMIF+celecoxib and celecoxib, respectively. (B) Histogram for S+G2/M phase of the cell cycle results in BPH-1 cells. Data are presented as mean±s.d., n =3. (C) Flow cytometry showed cell cycle in PWR-1E cells treated by control, rMIF, rMIF+celecoxib and celecoxib, respectively. (D) Histogram for S+G2/M phase of the cell cycle results in PWR-1E cells. Data are presented as mean±s.d., n =3. * P <0.05. Statistical analyses were performed using ANOVA followed by Tukey's test for multiple comparison.
Techniques Used: Flow Cytometry, Control, Comparison
Figure Legend Snippet: Expression of signaling molecules COX-2 and P53 involved in the inhibition of COX-2. (A) Western blotting detected the expression of COX-2 and P53 in BPH-1 cells treated by control, rMIF and rMIF+celecoxib, respectively. (B) Western blot analysis: the expression of COX-2 and P53 in BPH-1 cells treated by control, rMIF and rMIF+celecoxib. Data are presented as mean±s.d., n =3. (C) Western blotting detected the expression of COX-2 and P53 in PWR-1E cells treated by control, rMIF and rMIF+celecoxib, respectively. (D) Western blot analysis: the expression of COX-2 and P53 in PWR-1E cells treated by control, rMIF and rMIF+celecoxib. Data are presented as mean±s.d., n =3. * P <0.05. Statistical analyses were performed using ANOVA followed by Tukey's test for multiple comparison.
Techniques Used: Expressing, Inhibition, Western Blot, Control, Comparison
Related Articles
MTT Assay:Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: Fifteen thousand BPH-1 and PWR-1E cells were plated in each well of 96-well plates, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: One hundred thousand BPH-1 and PWR-1E cells were seeded into each well of a six-well plate, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: BPH-1 cells and PWR-1E cells were plated on 96-well plates (1000 cells per well) overnight and CCK-8 Assay:Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: Fifteen thousand BPH-1 and PWR-1E cells were plated in each well of 96-well plates, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: One hundred thousand BPH-1 and PWR-1E cells were seeded into each well of a six-well plate, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: BPH-1 cells and PWR-1E cells were plated on 96-well plates (1000 cells per well) overnight and Control:Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: Fifteen thousand BPH-1 and PWR-1E cells were plated in each well of 96-well plates, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: One hundred thousand BPH-1 and PWR-1E cells were seeded into each well of a six-well plate, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: BPH-1 cells and PWR-1E cells were plated on 96-well plates (1000 cells per well) overnight and Comparison:Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: Fifteen thousand BPH-1 and PWR-1E cells were plated in each well of 96-well plates, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: One hundred thousand BPH-1 and PWR-1E cells were seeded into each well of a six-well plate, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: BPH-1 cells and PWR-1E cells were plated on 96-well plates (1000 cells per well) overnight and Flow Cytometry:Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: Fifteen thousand BPH-1 and PWR-1E cells were plated in each well of 96-well plates, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: One hundred thousand BPH-1 and PWR-1E cells were seeded into each well of a six-well plate, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: BPH-1 cells and PWR-1E cells were plated on 96-well plates (1000 cells per well) overnight and Western Blot:Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: Fifteen thousand BPH-1 and PWR-1E cells were plated in each well of 96-well plates, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: One hundred thousand BPH-1 and PWR-1E cells were seeded into each well of a six-well plate, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: BPH-1 cells and PWR-1E cells were plated on 96-well plates (1000 cells per well) overnight and Expressing:Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: Fifteen thousand BPH-1 and PWR-1E cells were plated in each well of 96-well plates, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: One hundred thousand BPH-1 and PWR-1E cells were seeded into each well of a six-well plate, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: BPH-1 cells and PWR-1E cells were plated on 96-well plates (1000 cells per well) overnight and Inhibition:Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: Fifteen thousand BPH-1 and PWR-1E cells were plated in each well of 96-well plates, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: One hundred thousand BPH-1 and PWR-1E cells were seeded into each well of a six-well plate, and Article Title: The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling Article Snippet: BPH-1 cells and PWR-1E cells were plated on 96-well plates (1000 cells per well) overnight and |